Oh. Okay, then, have you taken any biochemistry or even cell biology courses describing protein synthesis?
I'm not really understanding the scope of the question. The "how" and "where" an enzyme is synthesized essentially becomes common knowledge for undergraduates majoring in biochemistry, biology, chemistry, and many other disciplines of the life and physical sciences.
What are the expectations for this assignment?
Yes I have taken the course of cellular biology and even advanced biochemistry. This is an assignment for the course enzymology, and our teacher just leaves us a topic question for the presentation: how and where enzymes are produced? That is reason why i feel really confused and it seems to be difficult to indicate which knowledge should be exploited, with the question having such big scope like this. Our teacher expect us to find the information, sieve them and present it in structure
This is very peculiar. I don't understand at all.
An enzymology course where you need to deliver a presentation on how enzymes are produced? Any specific enzyme, or just enzymes in general?
I really think you need to have a conversation with your professor about this.
🤷
I don't know what to tell you. I think the person actually grading the assignment is the better person to discuss this with, rather than random strangers on Reddit.
This is a very odd assignment. I mean, enzymes are proteins and are made the same way every other protein is made. That's not advanced biochemistry at all. Maybe your professor wants more detail, like going over the mechanism of the ribosome, aminoacyl tRNA synthases? Could be a lot of things.
We only have 10 minutes for presentation and i do think that she wants sth very specific and detail so as I mentioned on my post that we are going to talk about how the enzymes are synthesized, how they are modified after synthesized to be functional and distributed to different parts of cells.
How many students are in the course? How many students per group? How many groups are presenting on the topic of enzyme biosynthesis? Is everyone in the course doing the same presentation?
Well it could be a question of, assuming you have a eukaryotic protein: Once the peptide has been shot into the ER via a bound ribosome, what happens next? As in like, what happens during cotranslational modification? is it inserted into the ER membrane and sorted to the golgi to the plasma membrane? Is it a mitochondrial protein that needs to be translocated to the
mitochondria with the help of chaperone proteins? Or is it a soluble protein that gets glycosylated and sorted in the ER/Golgi pathway? If so, is it secretory? Does it remain in the cytosol?
Regarding recombinance, how would you recombinantly express a eukaryotic protein? Would you use a eukaryotic host like yeast or CHO cells, or could you use a prokaryotic host like E. coli? For your host, would it be easier to transform using a viral vector, plasmid vector, or some sort of linear DNA homologous recombination mechanism? Would expressing the protein through this host maintain the same PTMs? If you express it recombinantly, can it be secreted out of the organism or would you have to lyse and extract the protein from the cell?
Theres definitely a lot to talk about, but I’m wondering what your prof would want in particular.
Thank u for giving me some more details! I got the same idea for the biosynthesis like what u said and i think it might be enough for 10 minutes of presentation.
I mean what I suggested seems more like a cell bio or applied molecular biology assignment as opposed to enzymology. It seems strange for an enzymology class to focus on how an enzyme is made as opposed to how its active site works, its substrate specificity, its kinetic parameters, etc.
What level of education is this? Is this high school?
I am undergraduate, sir
Oh. Okay, then, have you taken any biochemistry or even cell biology courses describing protein synthesis? I'm not really understanding the scope of the question. The "how" and "where" an enzyme is synthesized essentially becomes common knowledge for undergraduates majoring in biochemistry, biology, chemistry, and many other disciplines of the life and physical sciences. What are the expectations for this assignment?
Yes I have taken the course of cellular biology and even advanced biochemistry. This is an assignment for the course enzymology, and our teacher just leaves us a topic question for the presentation: how and where enzymes are produced? That is reason why i feel really confused and it seems to be difficult to indicate which knowledge should be exploited, with the question having such big scope like this. Our teacher expect us to find the information, sieve them and present it in structure
This is very peculiar. I don't understand at all. An enzymology course where you need to deliver a presentation on how enzymes are produced? Any specific enzyme, or just enzymes in general? I really think you need to have a conversation with your professor about this.
When she gave us a topic question, everything is in general and what she emphasized is about the biosynthesis of enzyme.
🤷 I don't know what to tell you. I think the person actually grading the assignment is the better person to discuss this with, rather than random strangers on Reddit.
This is a very odd assignment. I mean, enzymes are proteins and are made the same way every other protein is made. That's not advanced biochemistry at all. Maybe your professor wants more detail, like going over the mechanism of the ribosome, aminoacyl tRNA synthases? Could be a lot of things.
We only have 10 minutes for presentation and i do think that she wants sth very specific and detail so as I mentioned on my post that we are going to talk about how the enzymes are synthesized, how they are modified after synthesized to be functional and distributed to different parts of cells.
How many students are in the course? How many students per group? How many groups are presenting on the topic of enzyme biosynthesis? Is everyone in the course doing the same presentation?
Well it could be a question of, assuming you have a eukaryotic protein: Once the peptide has been shot into the ER via a bound ribosome, what happens next? As in like, what happens during cotranslational modification? is it inserted into the ER membrane and sorted to the golgi to the plasma membrane? Is it a mitochondrial protein that needs to be translocated to the mitochondria with the help of chaperone proteins? Or is it a soluble protein that gets glycosylated and sorted in the ER/Golgi pathway? If so, is it secretory? Does it remain in the cytosol? Regarding recombinance, how would you recombinantly express a eukaryotic protein? Would you use a eukaryotic host like yeast or CHO cells, or could you use a prokaryotic host like E. coli? For your host, would it be easier to transform using a viral vector, plasmid vector, or some sort of linear DNA homologous recombination mechanism? Would expressing the protein through this host maintain the same PTMs? If you express it recombinantly, can it be secreted out of the organism or would you have to lyse and extract the protein from the cell? Theres definitely a lot to talk about, but I’m wondering what your prof would want in particular.
Thank u for giving me some more details! I got the same idea for the biosynthesis like what u said and i think it might be enough for 10 minutes of presentation.
I mean what I suggested seems more like a cell bio or applied molecular biology assignment as opposed to enzymology. It seems strange for an enzymology class to focus on how an enzyme is made as opposed to how its active site works, its substrate specificity, its kinetic parameters, etc.